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USLC Columns—Ultra Selective Liquid Chromatography

USLC Columns—Ultra Selective Liquid Chromatography

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Choose Columns Fast. Develop Methods Faster.

  • Represents the widest range of reversed phase selectivity available, with just 4 stationary phases: Aqueous C18, IBD, Biphenyl, and PFP Propyl.
  • Simplifies column choice for fast, effective method development.
  • Offers greater control to more easily resolve closely eluting peaks.
  • Maximizes column screening efficiency by ensuring orthogonality between analytical runs.
  • Fits universally on any HPLC or UHPLC instrumentation.

Selectivity is the most influential factor affecting peak separation, or resolution. Restek Ultra Selective Liquid Chromatography (USLC) technology is the directed application of orthogonal selectivity to help the practicing chromatographer develop methods more efficiently and more effectively. The Restek USLC column set offers the widest range of selectivity in the industry using just 4 unique stationary phases—Aqueous C18, IBD, Biphenyl, and PFP Propyl—to simplify phase selection.

Restek USLC columns are available on two silica lines to accommodate any instrument platform or chromatographic application. Pinnacle DB columns (1.9 µm) feature base deactivated silica and are recommended for scalable HPLC and UHPLC analyses. Ultra columns (3 and 5 µm) use highest purity silica and are well suited for traditional HPLC analyses.

USLC Method Development Toolbox

All the Right ToolsAll in One Toolbox

  • Ultra Selective Liquid Chromatography (USLC) method development toolbox contains all four USLC stationary phases in one convenient package.
  • Available for UHPLC (1.9 μm) and HPLC (3 or 5 μm) in 50, 100, or 150 mm lengths.

Pinnacle DB Biphenyl Columns (USP L11)

Stationary Phase Category: phenyl (L11)
Ligand Type: unique Biphenyl
Particle: 1.9 µm, 3 µm, or 5 µm, spherical
Pore Size: 140 Å
Carbon Load: 8%
End-Cap: yes
Surface Area: 150 m2/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Ultra Biphenyl Columns (USP L11)

Stationary Phase Category: phenyl (L11)
Ligand Type: unique Biphenyl
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 15%
End-Cap: yes
Surface Area: 300 m2/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Pinnacle DB PFP Propyl Columns (USP L43)

Stationary Phase Category: fluorophenyl propyl (L43)
Ligand Type: pentafluorophenyl propyl
Particle: 1.9 µm, 3 µm, or 5 µm, spherical
Pore Size: 140 Å
Carbon Load: 6%
End-Cap: yes
Surface Area: 150 m2/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Ultra PFP Propyl Columns (USP L43)

Stationary Phase Category: fluorophenyl propyl (L43)
Ligand Type: pentafluorophenyl propyl
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 11%
End-Cap: yes
Surface Area: 300 m2/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Pinnacle DB Aqueous C18 Columns (USP L1)

Stationary Phase Category: modified C18 (L1)
Ligand Type: proprietary polar modified and functionally bonded C18
Particle: 1.9 µm, 3 µm, or 5 µm, spherical
Pore Size: 140 Å
Carbon Load: 6%
End-Cap: no
Surface Area: 150 m2/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Ultra Aqueous C18 Columns (USP L1)

Stationary Phase Category: modified C18 (L1)
Ligand Type: proprietary polar modified and functionally bonded C18
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 15%
End-Cap: no
Surface Area: 300 m2/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Pinnacle DB IBD UHPLC Columns (USP L68)

Stationary Phase Category: polar-embedded alkyl (L68)
Ligand Type: proprietary polar functional embedded alkyl
Particle: 1.9 µm, spherical
Pore Size: 140 Å
Carbon Load: proprietary
End-Cap: no
Surface Area: 150 m2/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Ultra IBD Columns (USP L68)

Specialized Columns for Mixed Polar and Nonpolar Compounds

Stationary Phase Category: polar-embedded alkyl (L68)
Ligand Type: proprietary polar functional embedded alkyl
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 12%
End-Cap: no
Surface Area: 300 m2/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

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USLC Columns Put the Right Tools in Your LC Method Development Toolbox - Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection

Column selectivity has the most significant influence on chromatographic peak separation, or resolution, so choosing the right column can greatly speed up HPLC and UHPLC method development. In this article, we discuss column choice and identify a set of just 4 stationary phases—Restek’s USLC column set—that encompasses the widest range of reversed phase selectivity available today.

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Learn what de-wetting is, and how to avoid it, by using an Aqueous C18 column, instead of a conventional C18, when analyzing polar compounds by HPLC.

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USLC Columns: Choose Columns Fast. Develop Methods Faster.

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USLC Column Selection & Mobile Phase Adjustment Guide

USLC columns and this guide will help you easily choose the right stationary phase to target nearly any analyte in reversed phase or HILIC method development. It will also help you adjust your mobile phase to further improve results without guesswork or wasted time.

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