Weight Loss Drugs on Force Biphenyl

PeakstR (min)Positive MRMNegative MRM
1.Phentermine1.78150.1/133.2-
2.Topiramate2.88-338.1/78.0
image
LC_PH0533
ColumnForce Biphenyl (cat.# 9629312)
Dimensions:100 mm x 2.1 mm ID
Particle Size:3 µm
Pore Size:100 Å
Temp.:30 °C
Sample
Diluent:Water:acetonitrile (90:10)
Conc.:50 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:Water, 0.1% formic acid
B:Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.59010
4.000.51090
4.500.51090
4.600.59010
6.000.59010
DetectorMS/MS
Mode:MRM
InstrumentUHPLC
NotesThe detector is operated in multi-period mode. Period 1, from 0.0 to 2.4 min, is ESI+ mode, and Period 2, from 2.4 to 6.0 min, is ESI- mode.
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