The Analysis of Morphine & Metabolites in Urine without Hydrolysis on Force Biphenyl

PeakstR (min)Precursor IonProduct IonProduct Ion
1.Morphine-3-β-D-glucuronide0.61462.1286.0152.1
2.Morphine1.06286.1152.0164.9
3.Morphine-6-β-D-glucuronide1.17462.1286.0152.1
4.Morphine-N-oxide1.30302.1284.9161.9
5.6-Monoacetylmorphine1.81328.1211.1165.0
6.Hydrocodone1.91300.3199.2128.3
image
LC_CF0680
ColumnForce Biphenyl (cat.# 9629252)
Dimensions:50 mm x 2.1 mm ID
Particle Size:1.8 µm
Guard Column:UltraShield UHPLC precolumn filter, 0.2 µm frit (cat.# 25809)
Temp.:40 °C
Sample
Conc.: A 750 ng/mL standard was prepared in 1 mL of urine. 100 μL of the fortified urine standard was diluted with 900 μL of 0.1% formic acid in water prior to analysis.
Inj. Vol.:2 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in methanol
Time (min)Flow (mL/min)%A%B
0.000.58515
0.500.58515
2.000.53070
2.010.58515
4.000.58515
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC
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