Water Soluble Vitamins on Ultra Aqueous C18

PeaksConc.
(mg/mL)
1.Thiamin (B1)250
2.Ascorbic acid (C)1,000
3.Unknownn/a
4.Nicotinic acid (B3)1,000
PeaksConc.
(mg/mL)
5.Unknownn/a
6.Pantothenic acid (B5)1,000
7.Folic acid (B9)500
8.Riboflavin (B2)250
9.Methylparaben0.2
image
LC_0141
ColumnUltra Aqueous C18 (cat.# 9178575)
Dimensions:250 mm x 4.6 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:27 °C
Samplewater soluble vitamins
Diluent:initial dilutions of B1 and B2 basified with ammonium hydroxide
Conc.: variable
Mobile Phase
A:25 mM potassium phosphate, pH 2.00: methanol (95:5)
B:methanol: 25 mM potassium phosphate, pH 3.5 (60:40)
Time (min)%B
0-60
6.0125
11100
16100
Flow:1 mL/min
DetectorUV/Vis @ 254 nm
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B vitamins

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