delim
The Restek Biphenyl: Leading Resolution in LC since 2005

  • Separates a wider range of compounds than other phenyl and C18 chemistries.
  • Increases sensitivity and selectivity in LC-MS while using simple, MS-friendly mobile phases.
  • Restek’s most popular LC phase—available on traditional FPP and SPP core-shell silica.
The Restek Biphenyl - Leading Resolution in LC

Launched in 2005, the innovative Biphenyl (USP L11) is Restek’s most popular LC stationary phase because it is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl chemistries. As a result, rugged Biphenyl columns are extremely useful for fast separations—even with isobars—in drug, metabolite, and other analyses, especially those that require a mass spectrometer (MS). Increasing retention of early-eluting compounds can limit ionization suppression, and the Restek Biphenyl's heightened selectivity helps eliminate the need for complex mobile phases that are not well-suited for MS detection.

With ten years of unrivaled performance, Restek's time-tested Biphenyl has been proven to be the ideal column for clinical diagnostic, pain, pharma, and environmental labs seeking universal utility for mass spec applications. Restek was the first to bring you the benefits of the Biphenyl ligand, and our LC group has the veteran technical expertise to supply and support your lab better than anyone else.


The Restek Biphenyl is the Right LC Column in Any Format:

FEATURED
Raptor SPP Columns: A new species of column that combines the speed of superficially porous particles (SPP or “core-shell”) with time-tested Restek Biphenyl selectivity.


Force FPP Columns: Long-lasting, reproducible, and fully scalable FPP (fully porous particle) columns that let you easily transfer methods across your entire lab, from trusted-but-aged HPLCs to cutting-edge UHPLCs.


ALSO AVAILABLE
Ultra Columns: High-purity, type-B silica that minimizes activity and creates high-density bonding for reliable HPLC use.

Viva Columns: Rugged, spherical particles with a wide 300 Å pore size and narrow distribution that are designed for large-molecule separations.

DECONSTRUCTING THE BIPHENYL

With USLC technology, Restek's LC experts have defined our columns' selectivity based on molecular interactions. The more rings shown, the more significant a role played in solute retention. Learn more at www.restek.com/uslc

Properties:

  • Increased retention for dipolar, unsaturated, or conjugated solutes.
  • Enhanced selectivity when used with methanolic mobile phase.
  • Ideal for increasing sensitivity and selectivity in LC-MS analyses.

Switch to Biphenyl when:

  • Limited selectivity is observed on a C18.
  • You need to increase retention of hydrophilic aromatics.

Raptor Biphenyl LC カラム (USP L11)

  • 薬物や代謝物分析といった生化学分野のアプリケーションに最適です。
  • C18や他のフェニルカラムでは溶出が早すぎたり、分離が難しい化合物に対する選択性や保持が向上。
  • イオンサプレッションを回避し、シンプルで質量分析計に優しい移動相が可能に。
  • Restek のコアシェルタイプRaptor LC カラム の粒子径サイズは1.8、2.7および5µm

Force Biphenyl LC カラム (USP L11)

・ 薬物や代謝物分析など、バイオアナリシスアプリケーションに最適。
・ C18や他のフェニル系カラムでは分離や保持が難しい化合物に対し、選択性と保持が強まる。
・ 質量分析計に適した移動相が使用できる。
・ Restekで最も定評のある固定相。

Ultra Biphenyl カラム (USP L11)

粒子径:3 μmまたは5 μm、球状
細孔径:100Å
炭素含有率:15%
エンドキャップ: あり
pH範囲:2.5~8.0
温度上限: 80℃
USPコード:L11
固定相カテゴリー:Phenyl
リガンドタイプ:Biphenyl

Ultra Biphenyl Prep カラム

Our broadest selection of stationary phases, including unique phases. High-density bondings for maximum retention. High-purity, Type-B silica gives excellent peak shapes for a wide range of compounds.

Viva Biphenyl カラム (USP L11)

Stationary Phase Category: phenyl (L11)
Ligand Type: Biphenyl
Particle: 5 µm, spherical
Pore Size: 300 Å
Carbon Load: 7%
End-Cap: yes
Surface Area: 100 m2/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

USLC Method Development Toolbox

All the Right Tools—All in One Toolbox

  • Ultra Selective Liquid Chromatography (USLC) method development toolbox contains all four USLC stationary phases in one convenient package.
  • Available for UHPLC (1.9 μm) and HPLC (3 or 5 μm) in 50, 100, or 150 mm lengths.

EXP ダイレクト コネクト ホルダー

  • 工具不要の手締めタイプなので、締めすぎて接続箇所を破損したりせずに簡単にカートリッジ交換ができます。
  • 特許取得のチタンハイブリッドフェラルは、高圧下でのシール性を損なうことなく、繰り返し使用できます。
  • 自動調節可能なZDV(ゼロデットボリューム)により、どんなタイプの10-32メスポートでも接続できます。
  • EXPダイレクトコネクトホルダーには、別途ガードカラムカートリッジが必要です:内径は2.1、3.0及び4.6mmがあります。

Raptor EXP ガードカラムカートリッジ

  • カートリッジは手締めで簡単に交換できます。工具は必要ありません。
  • チタンハイブリッドフェラルは、高圧シールを損なうことなく繰り返し装着することができます。
  • 接続は汎用的な10-32ポートで、自動調整のゼロデッドボリューム接続が可能です。

Force EXPガードカラムカートリッジ

・ 移動相の配管を外さずにカートリッジ交換ができます。手締めタイプで工具も必要ありません。
・ 特許取得のチタンハイブリッドフェラルは高圧下でのシール性を損なうことなく再利用可能です。
・ 自動調整機能により、あらゆる10-32 Female portに対しZDV(Zero dead volume)接続ができます。

HPLC & UHPLC用EXP フィッティング 10-32フィッティング、 1/16インチ配管用

EXPハンドタイトフィッティング

  • 手締めのフィッティングは、HPLCの配管接続が容易です。8,700 psi(60 MPa)までのシールであれば工具は必要ありません。
  • ハンドタイトとヘックスヘッドスタイルのレンチは、UHPLCで用いられる 20,000 psiまでのシールが可能です。
  • 特許取得済みのフェラルは、高圧条件下で締め付けても繰り返し使用することができます。
  • フェラルはチタンの耐久性とPEEKのシール性能を組み合わせたハイブリットデザインです。
  • 最先端のフェラルが10-32メスポートにZDV(ゼロデッドボリューム)接続を提供します。ウォーターズタイプやパーカータイプなどカラムの接続様式を問いません。
  • 1/16 インチのPEEKおよびステンレス配管に適合。

Ultra ガードカートリッジ

Particle size: 5 µm spherical
Pore size: 100 Å

For a detailed description of the stationary phase in this guard cartridge, see the product page for an analytical column with the same silica and stationary phase (e.g., Ultra C18).

Viva ガードカートリッジ

Particle size: 5 µm spherical
Pore size: 300 Å

For a detailed description of the stationary phase in this guard cartridge, see the product page for an analytical column with the same silica and stationary phase (e.g., Viva C18).

UltraShield UHPLC プレカラム フィルター

  • Cost-effective protection for UHPLC systems.
  • Reliable way to filter out particulates and extend column lifetime.
  • Minimize extra column volume and maximize UHPLC sample throughput vs. guard cartridges.
  • Universal fitconnects easily to any brand column.
  • Leaktight to 15,000 psi (1,034 bar).
  • 0.5 µm or 0.2 µm stainless steel frit in a stainless steel body with PEEK ferrule.
臨床、法医、中毒学

Overcoming the Effects of Highly Organic Protein Precipitation Extracts on LC Peak Shape Using Direct Injection

Protein precipitation is frequently used to minimize matrix impact when analyzing biological samples. However, the effects of highly organic protein precipitation sample extracts on LC peak shape can negatively impact accurate quantification. Dilution or further sample preparation steps are often used to minimize these effects; however, here we show that direct injection of sample extracts is a viable option that can be used to prevent peak distortion, while avoiding the time and variability associated with additional sample preparation.

臨床、法医、中毒学

LC-MS/MSを用いた全血中免疫抑制剤のハイスループット分析

臓器移植の成功は、免疫抑制剤の正確かつタイムリーな分析に大きく依存します。本検討では、全血中のシクロスポリンA、タクロリムス、シロリムスおよびエベロリムスの迅速かつ正確な方法を開発しました。ここでは、Raptor Biphenylカラムを用いたLC-MS/MS分析とタンパク質沈殿による前処理のみを組合わせ、マトリックスの影響を受けずに、3分という高速分析をおこなうことができました。直線性、堅牢性、精確さおよび精度において優れた結果が得られ、この方法が治療薬物モニタリングにおけるハイスループット分析に適していることが示されました。

臨床、法医、中毒学

LC-MS/MSによる231種類の薬物およびその代謝物のための分析法の開発

薬物分析の需要が増加するにつれ、多くのラボが時間短縮や感度、特異性を向上させるためにLC-MS/MS法に取り組みつつあります。ここに示す方法では、Raptor Biphenylカラムを使用しているため、231種類の薬物およびその代謝物の迅速かつ正確な分析が可能となりました。

製薬

Novel Column Chemistry—High Impact, Low Cost Technology

Novel column chemistries are a simple change in an already budgeted consumable that can lead to optimized and more reliable methods—giving a fast return on a minimal investment.

論説

The Role of Selectivity in Liquid Chromatography Method Development

Dr. Schug, Associate Professor and Shimadzu Distinguished Professor of Analytical Chemistry in the Department of Chemistry and Biochemistry at The University of Texas at Arlington, discusses the role of column selectivity in liquid chromatography method development.

食品、香料、フレーバー

Increase Data Quality for Sulfonamide Residue Analysis by HPLC and UHPLC Using Unique Biphenyl Column Selectivity

The unique selectivity and high retention of Biphenyl columns produce complete separation of sulfonamide residues and benefit both UV and MS detection. In addition, Biphenyl columns in a UHPLC format allow faster sample throughput when testing for these antibiotics.

生体分析

A Rapid and Sensitive LC-MS/MS Method for the Analysis of Three Forms of Thyroid Hormones Using Raptor Biphenyl LC Columns

The Raptor Biphenyl column is excellent for rapid and sensitive analysis of thyroid hormones. With the method described here, concentrations of thyroid hormones as low as 2 pg/mL (T3) or 5 pg/mL (T4 and rT3) can be determined with less than 3.5 minutes of total analysis time. The analytical method is thus applicable to the clinical analysis of free thyroid hormone at low pg/mL levels.

PDF Document

Raptor Biphenyl LC Columns Brochure

Raptor LC columns combine the speed of superficially porous particles (i.e., SPP or “core-shell”) with the resolution of highly selective USLC technology. Featuring Restek's most popular LC stationary phase, the rugged Raptor Biphenyl is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). (PDF - 6MB)

PDF Document
PDF Document
PDF Document

Raptor Biphenyl LC Columns Sales Sheet

Fast, Rugged Raptor Columns with Time-Tested Selectivity: The industry-leading Biphenyl, first introduced by Restek in 2005, is our most popular LC stationary phase because it is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl chemistries. As a result, the rugged Raptor Biphenyl column is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). (PDF - 4MB)

PDF Document

Force LC Columns Brochure

Force LC columns are long-lasting and reproducible, even under the stress of UHPLC. Premium quality is backed by the strength of our 100% Pure Satisfaction guarantee. And perhaps most important, Force LC columns are fully scalable between 3 or 5 µm HPLC and 1.8 µm UHPLC, so you can easily transfer and optimize methods without extensive calculations. (PDF - 0MB)

PDF Document