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Frequently Asked Questions (FAQs): Reference Standards

    Reference Standards

  1. How can I purchase a custom-formulated reference standard from Restek?

  2. Where can I find a Certificate of Analysis, Safety Data Sheet (SDS), or Data Pack for my stock or custom reference standard?

  3. Can you recertify or extend the expiration date of my reference standard?

  4. What levels of documentation are available with my reference standards?

  5. What is included in a Restek reference standard data pack?

  6. Are your reference standards weight corrected for purity?

  7. What is the expiration date of my reference standard?

  8. How should I properly store my reference standards?

  9. What are certified reference materials (CRMs)?

  10. How do I know whether the reference standard I purchased from Restek is a certified reference material (CRM) as defined by ISO?

  11. Are my certified reference materials (CRMs) NIST traceable?

  12. Where can I find more information about Restek reference standards?

    Gas Reference Standards

  1. What do I do with my empty reusable gas cylinders?

  2. Can my gas standard be recertified?

  3. What regulator should I use with my gas standard?

  4. How can I troubleshoot pressure problems in my gas standard?

If your question doesn’t appear on the list, please contact Restek’s expert chemists.
(Remember to include your company name and complete mailing address.)




Reference Standards


1. How can I purchase a custom-formulated reference standard from Restek?

Because of the sheer number of compounds, solvents, and concentrations that can be uniquely combined into a reference standard, it is not uncommon for our customers to need a mix that we do not have readily available as a stock product. If you find yourself in that situation, simply submit a custom reference standard request using our easy online tool. Our reference standards group will evaluate your request for feasibility and pricing, assign a quote number, and deliver the finished quotation to you—typically within three business days. Once you receive a response, give your quote number to Restek Customer Service or your local Restek representative to place an order; we will generate a custom catalog number just for you and will prepare the formulation. Restek even offers standing orders (also known as “blanket orders”) on our custom standards to drastically lower your cost!

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2. Where can I find a Certificate of Analysis, Safety Data Sheet (SDS), or Data Pack for my stock or custom reference standard?

Use our reference standard documentation search tool to find certificates of analysis, safety data sheets (SDS), or data packs for your Restek reference standards, whether stock or custom. For stock products, you can also access documentation for the most recently produced lots through the item's product page on the Restek website—simply enter the cat.# into the main site search tool (found in the upper right corner of every page), and then, once you're on the product page, click on the tab labeled “Documentation.” For custom products or older lots of stock products, you must enter the cat.# and lot number into our Documentation Search tool.

If you cannot find a particular certificate, SDS, or data pack online, feel free to e-mail standards@restek.com

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3. Can you recertify or extend the expiration date of my reference standard?

Unfortunately, no. Restek conducts real-time stability studies and accelerated stress tests to establish reliable expiration dates. Use beyond the identified expiration date is neither recommended nor guaranteed.

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4. What levels of documentation are available with my reference standards?

Nearly all of our stock reference standards are provided with the highest level of documentation available—quantitative (certificate of analysis)—and we provide three options for most of our custom reference standards. Reference standards that fall within our ISO scopes of accreditation will be classified as certified reference materials (CRMs) regardless of documentation level.

  • Gravimetric: Product supplied with the gravimetric records listing purity of each material used, calculated concentration, a unique product lot number, raw material lot numbers, and isomer ratios for isomeric compounds.

  • Qualitative (Certificate of Composition): A single sample withdrawn from the packaged units is tested by the appropriate technique to verify mixture composition. Product is supplied with a certificate of composition showing a chromatogram of the standard with each peak identified, along with its raw material purity, and gravimetric concentration.

  • Quantitative (Certificate of Analysis) A sample of the packaged unit is analyzed in triplicate and the peak areas are statistically compared to a previous lot (if available) or a second lot. A detailed data pack is available at www.restek.com/documentation containing gravimetric documentation, all quantitative assay raw data, the exact amount of each raw material used, the total volume prepared, and statistics. Test results for raw material purity and identification are available upon request.

For more information, see www.restek.com/pdfs/GNBR1845B-UNV.pdf

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5. What is included in a Restek reference standard data pack?

Our detailed data packs are designed to provide an exhaustive compendium of data describing your reference standard and feature gravimetric documentation, all quantitative assay raw data, and statistics. At a minimum, the following data sets are included:

  • Chemical Standard Lot Sheet showing the lot number, purity, and exact gravimetric weight of each analyte as well as the volume of solvent used in formulating the product.

  • Acceptance Chromatogram(s) showing elution order and relative peak sizes.

  • Data Analysis Spreadsheet based on the chromatogram(s).

  • Certificate of Analysis (CoA).

To obtain a data pack, visit www.restek.com/documentation. All raw material purity and identification results are available upon request.

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6. Are your reference standards weight corrected for purity?

Yes! Raw material purity information is recorded on the certificate of analysis. Restek’s Quality Control (QC) lab confirms the chemical identity and purity of mixture components and solvents using two or more of the following techniques: GC-FID, HPLC, GC-ECD, GC-MS, LC-MS, refractive index, and melting point. Compounds with a listed purity of less than 99% have been weight corrected to compensate for the presence of extraneous impurities in the compound, inorganic moieties (salts, hydrates, etc.), and organic moieties (typically the functional group of a derivatized compound). To better illustrate these scenarios, the following examples will explain the process we apply:

Example 1 – Purity adjustment

Compound A is tested and found to contain a 2% impurity (compound B). That is, the purity of compound A is 98%. So, if 100 mg is weighed out, the material will contain 98 mg of compound A and 2 mg of compound B. Adding 100 mL of solvent will produce a concentration of 980 µg/mL for compound A, not the desired 1,000 µg/mL. To reach a concentration of 1,000 µg/mL for compound A, Restek chemists apply a correction factor of 0.98 to obtain the final concentration:

100 mg ÷ 0.98 = 102 mg

  • Using the following calculation will result in 100 mg of compound A being added to the solution and the desired concentration of 1,000 µg/mL:

    (102 mg ÷ 100 mL) x 1,000 = 1,020 µg/mL x 0.98 = 1,000 µg/mL compound A

Example 2 – HCl adjustment

Compound A is the hydrochloride salt of an organic amine. If the molecular weight of compound A is 100 AMU, then the molecular weight of the parent compound (organic moiety) would be 63.5 AMU—molecular weight of compound A minus molecular weight of HCl. The ratio of organic moiety to inorganic moiety would be:

63.5 ÷ 100 = 0.635

If 100 mg is weighed out, the material will contain 63.5 mg of the parent compound and 36.5 mg of HCl. Adding 100 mL of solvent will produce a concentration of 635 µg/mL for the parent compound, not the desired 1,000 µg/mL. To reach a concentration of 1,000 µg/mL for the parent compound, Restek chemists apply a correction factor of 0.635 to obtain the final concentration:

100 mg ÷ 0.635 = 157.5 mg

  • Using the following calculation will result in 100 mg of compound A being added to the solution and the desired concentration of 1,000 µg/mL:

    (157.5 mg ÷ 100 mL) x 1,000 = 1,575 µg/mL x 0.635 = 1,000 µg/mL parent compound

Example 3 – DNPH adjustment

Compound A is the dinitrophenylhydrazone (DNPH) derivative of a carbonyl compound. If the molecular weight of compound A is 250 AMU, then the molecular weight of the parent compound (carbonyl moiety) would be 70 AMU—molecular weight of compound A minus molecular weight of DNPH moiety. The ratio of carbonyl moiety to carbonyl DNPH moiety would be:

70 ÷ 250 = 0.28

If 100 mg is weighed out, the material will contain 28 mg of the parent compound and 72 mg of DNPH. Adding 100 mL of solvent will produce a concentration of 280 µg/mL for the parent compound, not the desired 1,000 µg/mL. To reach a concentration of 1,000 µg/mL for the parent compound, Restek chemists apply a correction factor of 0.28 to obtain the final concentration:

100 mg ÷ 0.28 = 357.1 mg

  • Using the following calculation will result in 100 mg of compound A being added to the solution and the desired concentration of 1,000 µg/mL:

    (357.1 mg ÷ 100 mL) x 1,000 = 3,571 µg/mL x 0.28 = 1,000 µg/mL parent compound

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7. What is the expiration date of my reference standard?

The expiration date of an unopened ampul, stored under the recommended storage conditions, is identified on your certificate of analysis. (If only the month and year are given, the standard expires on the last day of the month.) The expiration date is also printed on the ampul label. Once opened, the integrity of the standard is subject to handling and storage conditions, and it cannot be guaranteed.

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8. How should I properly store my reference standards?

Restek reference standards are provided with documentation that states the recommended storage conditions needed to ensure the optimal stability of the components in that specific standard. Storage at lower-than-specified temperatures is acceptable unless otherwise noted on the certificate. (If the ampul is stored at lower temperatures, be sure to invert the ampul several times prior to opening. In the event of observable undissolved material, warm and sonicate the ampul before opening.)

Short-term exposures (≤ seven total days) to higher temperatures is acceptable and should not, based on our studies, negatively affect the long-term stability of your product. For specific statements and claims on product uncertainty as a result of short-term exposures to elevated temperatures, refer to the certified uncertainty value notes on each product’s certificate. In addition to possible product-specific information, the following notes will be included:

  • It is important to note that the shipping stability uncertainty was obtained under temperature extremes for specific time intervals; therefore, the certified combined stressed uncertainty value should only be applied to the product if it was stored at nonstandard temperature conditions up to and including seven days. Contact Restek Technical Service at support@restek.com for use recommendations if your shipment was in-transit for more than seven days at nonstandard temperature conditions.

  • Apply the certified combined unstressed uncertainty value if the product was received under standard shipping conditions. Apply the certified combined stressed uncertainty value if the product was received under nonstandard conditions as specified below:

    Label Conditions

    Standard Conditions

    Non-Standard Conditions

    25 °C Nominal (Room Temperature)

    < 60 °C

    ≥ 60 °C up to 7 days

    10 °C or Colder (Refrigerate)

    < 40 °C

    ≥ 40 °C up to 7 days

    0 °C or Colder (Freezer)

    < 25 °C

    ≥ 25 °C up to 7 days


Please note that once the product is opened, the integrity of the standard is subject to handling and storage conditions, and it cannot be guaranteed.

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9. What are certified reference materials (CRMs)?

A CRM from Restek is in an exclusive subset of reference standards that meets the following set of strict criteria defined under ISO 17034 and ISO/IEC 17025:

  • Made of raw materials characterized via qualified methods on qualified instruments.

  • Produced in an ISO-accredited lab under documented procedures.

  • Falls under the manufacturer’s scopes of accreditation.

To learn more about Restek’s ISO quality credentials and to view our certificates (including scopes of accreditation), visit www.restek.com/iso

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10. How do I know whether the reference standard I purchased from Restek is a certified reference material (CRM) as defined by ISO?

  • Before you purchase, you can check at www.restek.com/iso to see if your standard falls within our scope of accreditation. You can also ask Restek Customer Service or your local Restek representative.

  • After you purchase, you can easily confirm that your standard is a CRM by looking at your certificate of analysis (CoA). For a standard to be considered a CRM, it must be both manufactured and tested in an ISO-accredited lab. Every certified reference material we sell will have separate A2LA and A2LA/ILAC logos in the upper right-hand corner of its CoA, signifying that it was both manufactured and tested under our ISO 17034 and ISO/IEC 17025 accreditations. The CoA will also state "Certified Reference Material" at the top.

Please note that our gas reference standards are not within our scope of accreditation, so they are not certified reference materials (CRMs).

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11. Are my certified reference materials (CRMs) NIST* traceable?

Yes! Reference standards manufactured within our scopes of our accreditation (i.e., CRMs) are traceable as follows:

  • All analytical balances are verified at seven mass levels daily using NIST traceable weights and are also calibrated yearly by an ISO/IEC 17025:2005–accredited provider to guarantee accurate measurement.

  • Volume is assured by using Class A volumetric flasks and/or Class A pipettes.

  • Raw materials are characterized to confirm identity and purity using two or more of the following techniques: GC-FID, HPLC, GC-ECD, GC-MS, LC-MS, refractive index, and melting point.

  • Only the purest materials available are used; any material with purity less than 99% is weight corrected.

    * National Institute of Standards and Technology

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12. Where can I find more information about Restek reference standards?

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Gas Reference Standards


1. What do I do with my empty reusable gas cylinders?

Local regulations can vary greatly. If you wish to dispose of or recycle empty cylinders, be sure to do so according to local regulations. Some cylinders can be returned to the manufacturer; for more information, directly contact Spectra/Linde Gases, Inc. (1-800-932-0624) or Airgas (formerly Scott/Air Liquide gas) (1-800-331-4953).

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2. Can my gas standard be recertified?

Recertification is available for gas standards. Gas standards can be expensive and tend to be stored in the lab for months, if not years, so it is wise to have the composition recertified by a qualified vendor. For gas standards purchased from Restek, request recertification directly from Spectra/Linde Gases, Inc. (1-800-932-0624) or Airgas (formerly Scott/Air Liquide gas) (1-800-331-4953). For gas standards purchased from vendors other than Restek, it is necessary to contact the vendor who manufactured the mixture.

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3. What regulator should I use with my gas standard?

We offer six regulators for our gas standards. Specifications are summarized below:



DCG Partnership Cylinders:

Size: 7.6 x 24 cm

CGA-170/110 connection

U.S. DOT Specs: DOT-4B-240ET

Please note: This cylinder is not approved for use in Canada.

Recommended regulator:
cat.# 22032



Airgas 110 L
(Pi-marked Cylinders for EU Regulations):

Aluminum construction

Size: 8.3 x 29.5 cm

Volume/Pressure:
  110 liters of gas @ 1,800 psi

CGA-180 outlet fitting

Weight: 2.2 lb/1 kg

DOT Specifications: 3AL2216

Recommended regulator:
cat.# 26371, 26372, 21572, or 21572-R100




Spectra (Linde) 104 L:

Aluminum construction

Size: 8 x 24 cm

Volume/Pressure:
  104 liters of gas @ 1,800 psi

CGA-180 outlet fitting

Weight: 1.5 lb/0.7 kg

Recommended regulator:
cat.# 21572, 21572-R100, 26371, or 26372




Airgas 110 L:

Aluminum construction

Size: 8.3 x 29.5 cm

Volume/Pressure:
  110 liters of gas @ 1,800 psi

CGA-180 outlet fitting

Weight: 2.2 lb/1 kg

DOT Specifications: 3AL2216

Recommended regulator:
cat.# 26371, 26372, 21572, or 21572-R100




Airgas 14 L:

Contents: 14 liters

Pressure: 240 psig (17 bar)

Outlet Fitting: CGA 160

Weight: 1.5 lb/0.7 kg

Dimensions:
  3" diameter x 11" height
  (7.6 x 28 cm)

DOT Specifications: 4B240

Please note: This cylinder is not approved for use in Canada.

Recommended regulator:
cat.# 22690




Airgas 48 L:

Contents: 48 liters

Pressure: 300 psig (21 bar)

Outlet Fitting: CGA 165

Weight: 1.75 lb/0.8 kg

Dimensions:
   4" diameter x 16 ¼" height
   (10.2 x 41 cm)

DOT Specifications: 39 NRC

Please note: This cylinder is not approved for use in Canada.

Recommended regulator:
cat.# 22691



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4. How can I troubleshoot pressure problems in my gas standard?

The most common problem encountered with gas standards is the absence of a pressure reading at the regulator. To check your regulator, remove it from the cylinder, and connect it to a cylinder that you are sure has pressure. If the regulator registers a reading on the “good” cylinder, the problem is likely that the cylinder in question is empty. If the regulator does not register a reading on the “good” cylinder, then the problem is the regulator and it must be repaired or replaced.

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