QuEChERS Solutions

Even Easier

Comprehensive Solutions Simplify
Sample Prep and Analysis

  • New free-flowing salts in slim packs make extraction a snap.
  • Complete line of easy-to-use QuEChERS products, reference standards, and accessories.
  • Follow up sample prep with a wide range of analytical columns for both LC and GC.

Keep your workflow running smoothly with Q-sep extraction salts from Restek!

Pure Satisfaction
Pure Satisfaction

For years, the QuEChERS approach to sample preparation has been making the lives of food safety scientists easier. Gone are the days of time-consuming, solvent-intensive extraction techniques and multiple solid phase extraction cartridge cleanup steps! Research published by the U.S. Department of Agriculture Easter Regional Research Center in Wyndmoor, PA, [1] introduced to the world QuEChERS—a method that is Quick, Easy, Cheap, Effective, Rugged, and Safe.

With QuEChERS, a homogenized sample undergoes a quick extraction step where the analytes are driven into an organic solvent by the partitioning power of a blend of salts. After extraction, the sample is cleaned up through the use of a dispersive solid phase extraction (dSPE) step that is also quick and easy to perform. This simple, two-stage process offers significant savings in time, materials, and effort, making QuEChERS sample preparation faster and easier than other approaches.

Table I: Prepare samples more quickly, easily, and cost effectively with QuEChERS.


Mini-Like or Modified
Luke Method


with QuEChERS

Estimated time to process 6 samples (min)



4x faster

Solvent used (mL)



6-9x less solvent

Chlorinated waste (mL)



Safer, cheaper, greener

Glassware/specialized equipment

capacity for 200 mL, quartz wool,
funnel, water bath, or evaporator



Since its introduction, the QuEChERS technique has evolved to accommodate an expanding list of pesticides in an increasingly diverse list of foodstuffs. At Restek, we offer Q-sep products to cover the four major approaches to QuEChERS, along with a host of other items to help make your QuEChERS experience simple and successful. We carry a comprehensive line of sample prep supplies, reference standards, and LC and GC columns that will help make QuEChERS even easier, whether you are new to the approach or developing a method for a new sample matrix.

Which product do I choose?

QuEChERS is primarily a two-stage process, and you’ll select Q-sep products for both stages. Your method may have specific recommendations, but in case you’re not sure what will work, here some considerations when making your selection.

Stage 1: Extraction

The choice of which extraction technique to use is made principally by considering your analytes of interest. If you’re looking for compounds that aren’t pH sensitive, the original unbuffered method [1] will work great. However, if your analytes of interest are pH sensitive, you will want to select one of the buffered methods—notably, the official EN 15662 method [2], the mini-multiresidue approach [3], or the official AOAC 2007.01 method [4].

Restek’s Q-sep products for the two European approaches, the official EN 15662 method and the mini-multiresidue approach, are the same, so it becomes essentially a choice between a European-developed method and the AOAC method. Both are buffered to lower the pH to a range where most pesticides are stable, and the methods differ only in the specific buffering salts and their ratios. Either would be a good place to start for most analyses. There are a great many studies that highlight specific instances when one buffering technique may outperform another, so a little research into your particular area of study may yield practical suggestions on which extraction method to use.

Stage 2: Dispersive Solid Phase Extraction (dSPE) Cleanup

The next big choice comes during the selection of which dSPE cleanup product to use. Where the choice of the extraction product was driven largely by the analytes of interest, the choice of cleanup is based on the sample being tested. Restek Q-sep dSPE products are formulated with different sorbents in different ratios so that your dSPE choice can be tailored to the composition of your particular sample type (e.g., fatty, highly pigmented, etc.). Use Table II to learn what each sorbent removes and to help select the best dSPE product for your particular sample.

Table II: Visit the desktop version of to access this table.

Table II: Select your dSPE tube based on sample type to ensure you get the best sorbent blend for removing specific matrix interferences.

Method Sorbent Mass (mg)
MgSO4 PSA* C18-EC GCB** Vial Volume (ml)
Excess Water Sugars, fatty acids, organic acids, anthocyanine pigments Lipids, nonpolar interferences Pigments, sterols, nonpolar interferences
lettuce General fruits and vegetables (e.g., celery, head lettuce, cucumber, melon) AOAC 2007.01 150 50 -
- 2 26124
Original unbuffered, EN 15662, mini-multiresidue 150 25 - - 2 26215
AOAC 2007.01 1,200 400 - - 15 26220
Original unbuffered, EN 15662 900 150 - - 15 26223
avocado Foods with fats and waxes (e.g., cereals, avocado, nuts, seeds, and dairy) Mini-multiresidue 150 25 25 - 2 26216
- 150 - 50 - 2 26242
AOAC 2007.01 150 50 50 - 2 26125
AOAC 2007.01 1,200 400 400 - 15 26221
- 1,200 - 400 - 15 26244
- 900 150 150 - 15 26226
strawberries Pigmented fruits and vegetables (e.g., strawberries, sweet potatoes, tomatoes) Mini-multiresidue, EN 15662 150 25 - 2.5 2 26217
AOAC 2007.01 150 50 - 50
2 26123
AOAC 2007.01 1,200
400 400 400 15 26222
EN 15662 900 150 - 15 15 26224
General Purpose Highly pigmented fruits and vegetables (e.g., red peppers, spinach, blueberries) Mini-multiresidue, EN 15662 150 25 - 7.5 2 26218
AOAC 2007.01 150 50 50 50 2 26219
EN 15662 900 150 - 45 15 26225
- 900 300 - 150 15 26126
General Purpose General purpose (wide range of commodities, including fatty and pigmented fruits and vegetables) - 150 50 50 7.5 2 26243
- 900 300 300 45 15 26245

Note: No entry in the Method column refers to dSPE formulations not specifically included in one of the cited references. These products can be used to accommodate the various needs of specific matrices not directly met by the cited references.
*PSA = primary secondary amine exchange material
**GCB = graphitized carbon black

Restek is not able to provide copies of these documents.
[1] M. Anastassiades, S.J. Lehotay, D. Stajnbaher, F.J. Schenck, Fast and easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solid-phase extraction" for the determination of pesticide residues in produce. J. AOAC Int. 86 (2003) 412-431.
[2] EN 15662, Foods of Plant Origin—Determination of Pesticide Residues Using GC-MS and/or LC-MS/MS Following Acetonitrile Extraction/Partitioning and Clean-up by Dispersive SPE—QuEChERS method, 2008.
[3] QuEChERS-A Mini-Multiresidue Method for the Analysis of Pesticide Residues in Low-Fat Products, 2004.
[4] AOAC Official Method 2007.01, Pesticide Residues in Foods by Acetonitrile Extraction and Partitioning with Magnesium Sulfate, 2007.


Raptor ARC-18 LC カラム (USP L1)

  • 理想的なハイスループットLC-MS/MSアプリケーションを最低限のサンプル前処理で
  • 多成分一斉分析とより良い検出のためのバランスのとれた保持プロファイル
  • 保持やピーク形状を犠牲にせずに低pHの移動相の使用が可能
  • Restek のコアシェルタイプRaptor LC カラム の粒子径サイズは1.8、2.7および5µm

Rxi-5Sil MS カラム (フューズドシリカ)

微極性; Crossbond 1,4-bis(ジメチルシロキシ)フェニレン ジメチル ポリシロキサン

  • 低ブリードなGC-MS用カラム。
  • 活性のある化合物に対する優れた不活性度。
  • 汎用カラム-半揮発性化合物、多環芳香族化合物、有機塩素化合物、フタル酸エステル、フェノール類、アミン類、有機塩素系農薬、有機リン系農薬、薬物、溶媒中の不純物および炭化水素などのGC-MS分析に最適。
  • 使用温度範囲:-60 °C ~ 350 °C

Reference Standards

QuEChERS パフォーマンススタンダードキット

  • Designed for use in all QuEChERS methods for pesticides in fruits and vegetables, including the original unbuffered method, AOAC 2007.01, and EN 15662.
  • Kit contains organochlorine, organonitrogen, organophosphorus, and carbamate pesticides commonly used on fruits and vegetables.
  • Volatile, polar, active, base-sensitive, and nonvolatile compounds are included to allow comprehensive evaluation of QuEChERS extraction and cleanup efficiencies, and optimization of GC and LC instrumental conditions.
  • Ideal for initial method evaluations and ongoing method performance validations.
  • Analytes are divided into three ampuls based on compatibility for maximum stability and shelf life.*
  • Precise formulations improve data quality and operational efficiency; spend more time running samples and less time sourcing and preparing standards.
  • Quantitatively analyzed to confirm the composition and stability of each mixture.

    *When combining compounds with different functionalities, chemical stability can be an issue. The analytes in this kit are separated into three mixes to ensure maximum long-term storage stability. For analysis, a fresh working standard should be prepared by combining the three kit mixes in a 1:1:1 ratio to prepare a 100 µg/mL working standard solution. Once blended, Restek does not recommend storing working standards or subsequent dilutions for future use.

    Contains 1 mL each of these mixtures.
    31153: QuEChERS Performance Standard A
    31154: QuEChERS Performance Standard B
    31155: QuEChERS Performance Standard C

QuEChERS Internal Standards and Quality Control Standards

  • Single and multiple component standards as specified in EN Method 15662.
  • Support for GC and HPLC with MS, MS/MS, and selective detectors.
  • Ready to use for QuEChERS extractions—no dilutions necessary.

LC Multiresidue Pesticide Kit

  • Accurately detect and quantify pesticides of global food safety concern in a wide range of fruits, vegetables, and other commodities by LC-MS/MS.
  • Full kit contains 204 compounds of interest, covering many LC-determined pesticides listed by government agencies; individual ampuls also sold separately.

GC Multiresidue Pesticide Kit

  • Accurately identify and quantify pesticide residues by GC-MS/MS in fruits, vegetables, botanicals, and herbals such as tea, ginseng, ginger, echinacea, and dietary supplements.
  • Comprehensive 203-compound kit covers food safety lists by the FDA, USDA, and other global governmental agencies; individual ampuls also sold separately.

Sample Handling

Q-sep QuEChERS 抽出塩

  • 移し替えがきれいにおこなえる吸湿していないさらさらの塩です。
  • 抽出用チューブに直接入れられる開封しやすいパックのおかげで、余分な器具は不要です。
  • 便利なスリムパックはチューブにぴったりで、塩をこぼさず入れらます。
  • そのまま使えるチューブで、余分な器具は不要です。
  • 秤量済みの高純度な試薬です。
  • オリジナル法(バッファーなし)、AOAC法(2007.01)およびEN法 15662のQuEChERS法に最適です。

Q-sep QuEChERS 抽出液クリーンアップ用dSPE


  • 保護と保存安定性を高めるため、10個ずつホイルで個別包装しています。
  • すぐに使用可能なチューブで、ガラス器具は不要です。
  • 秤量済みの高純度な吸着剤。
  • オリジナル法(バッファーなし)、AOAC法(2007.01)、EN法 15662およびMini-Multiresidue法のQuEChERS法に対応しています。

Q-sep 3000 遠心分離機


  • 50 mL、15 mLおよび2 mLの遠心チューブに対応しています。
  • 設置スペースが小さくて済みます。
  • 安全で確実なUL、CSA、CE認証済み。1年保証付きです。

Q-sep ボトルトップ溶媒ディスペンサー

  • 調整ノブで1ストローク (0.5 mL刻み) あたり2.5 mL から 30 mL までの56段階の分注ができます。
  • ボトル取付け部分は30mmのスリードになっており、4つのアダプター (25 mm、28 mm、38 mmおよび45 mm)が付属しています。
  • ISO 8655規格に従って校正されており(証明書付き)、ユーザーによる再校正も可能です。
  • 化学薬品耐性に優れ、オートクレーブ可能なPTFE、ガラスおよびポリプロピレンでできています。
  • 安全放電で誤操作のリスクを減らし、ノズルキャップは試薬の液滴を防ぎます。
  • 清掃や点検も簡単です。


丈夫で洗浄が簡単なFEP(fluorinated ethylene propylene)製チューブは、通常の使用条件だと何回も使えてコストも50%下がります。

Q-sep チューブラック

  • 2 mL、15 mLおよび50 mL チューブ用があります。
  • サンプル位置がわかりやすいように、ラック上部に英数字を表示してあります。
  • 組み立ても簡単です。


Topaz 4.0 mm ID シングルテーパー 注入口ライナー

Agilent GCスプリット/スプリットレス注入口ライナー

Topaz GCインレットライナーの革新的技術とその不活性度は次のレベルへ
  • 不活性化処理—微量分析における真度と精度に重要な極めて低い分解性。
  • 再現性—様々な化合物に対する優れた信頼性を維持する厳しい製造管理と品質試験。
  • 不活性化処理—GC稼働率とスループットを最大限にあげる極めてクリーンなライナー。
  • 100%の満足度ーライナーの性能がお客様の期待にそぐわない場合、交換もしくは返品に対応します。


Evaluation of Dispersive and Cartridge Solid Phase Extraction (SPE) Cleanups for Multiresidue Pesticides in QuEChERS Extracts of Finished Tobacco Using GCxGC-TOFMS

We compared the efficacy of dSPE and cSPE cleanup methods for multiresidue pesticide analysis of QuEChERS extracts of finished tobacco products using GCxGC-TOFMS. Cleanup with dSPE tubes containing 7.5 mg of GCB and 25 mg of PSA produced better results than other methods, with average recoveries of 92% and 91% for 500 ppb and 50 ppb fortifications respectively.


Comparing Pesticide Residues in Amish and Commercially Grown Strawberries and Spinach Using QuEChERS, Various dSPE Sorbents, and GC-TOFMS

QuEChERS extraction, dSPE cleanup, and GC-TOFMS analysis were used to assess pesticide residue levels in strawberry and spinach from both commercial and Amish growers. Various dSPE sorbent products were compared to determine which were most effective.

PDF Document

Determining Pesticides in Dietary Supplements with QuEChERS Extraction, Cartridge SPE, and GCxGC-TOFMS

The novel approach used here combines QuEChERS extraction, cartridge SPE cleanup, and GCxGC-TOFMS analysis, resulting in good recoveries for a wide range of pesticides in dietary supplements. Matrices include dandelion root, sage, and a multi-herb finished product. (PDF - 0MB)

PDF Document

Mitigating Matrix Effects: Examination of Dilution, QuEChERS, and Calibration Strategies for LC-MS/MS Analysis of Pesticide Residues in Diverse Food Types

LC-MS/MS is popular for monitoring pesticide residues in food due to its selectivity and sensitivity; however, matrix effects can cause poor data quality and difficult quantification. We evaluated the relative effectiveness of sample dilution, QuEChERS cleanup, solvent-based calibration, and matrix-matched calibration strategies to mitigate matrix effects in celery, kale, avocado, lime, and brown rice flour. (PDF - 0MB)

PDF Document

QuEChERS Made Even Easier: Comprehensive Solutions Simplify Sample Prep and Analysis

Learn how to use the QuEChERS approach to perform faster, easier sample preparation and cleanup for multiresidue pesticide analysis. Q-sep QuEChERS products save time, money, and materials compared to modified Luke methods. (PDF - 0MB)

PDF Document

Developing New Methods for Pesticides in Dietary Supplements

QuEChERS is a simple, effective approach to sample prep that can be applied to the analysis of pesticides in dietary supplements. Here, we demonstrate a QuEChERS, cSPE, GC-TOFMS procedure that results in good recoveries for a wide range of pesticides in dandelion root. (PDF - 0MB)

PDF Document
PDF Document

Fast, Simple QuEChERS Extraction and Cleanup of Pesticide Residue Samples

QuEChERS dSPE methods simplify extract cleanup, reduce material costs, and improve sample throughput. Here we demonstrate the effectiveness of QuEChERS sample cleanup using a multiresidue analysis of pesticides on strawberries. (PDF - 0MB)

PDF Document

Fast Screening of Recalled Tylenol for Tribromoanisole and Related Adulterants

A recall of Tylenol pain reliever and other related products highlights the need for simple, quick sample preparation and a comprehensive analytical method for adulterants in consumer products. The rush to examine a multitude of samples in a short period of time is a common scenario for potential recalls, especially when a contaminant is found in a given product and rapid determinations need to be made to assess how widespread the problem may be. (PDF - 0MB)

PDF Document

How Column Inertness Improves the Chromatography of Basic Compounds

Not all column deactivations are appropriate for analyzing basic compounds. Here, we demonstrate the effect of column inertness on peak shape and discuss its role in improving method accuracy, sensitivity, and development time. (PDF - 0MB)

Audio Slideshow

Introduction to QuEChERS

A brief overview of the QuEChERS process, and how it can simplify your sample preparation workflow.

Multimedia On-Demand Webinars

Sample Preparation of Cannabinoids in Urine Using the QuEChERS Method and dSPE Cleanup

This webinar introduces QuEChERS and dSPE and discusses their application to sample prep for GC/MS analysis of cannabinoids in urine. Attendees will learn how to increase sample throughput with this simple approach.

Narrated by . Length: 16 minutes

Analysis of Veterinary Drug Residues using a Unique HPLC Biphenyl Stationary Phase

Analysis of veterinary drug residues in milk using a QuEChERS approach and biphenyl column is discussed. Better retention is achieved, compared to C18 or phenyl phases, improving separation of analytes and matrix components.

Narrated by . Length: 24 minutes